Journal: Journal of Neurochemistry

Article Title: Synaptobrevin‐2 Containing Extracellular Vesicles Are Rapidly Incorporated Into Mammalian Neurons via a Dynamin‐Dependent Pathway

doi: 10.1111/jnc.70551

Figure Lengend Snippet: Syb2‐containing neuronal EV uptake in the somatodendritic compartment depends on Dynamin and occurs independently of macropinocytosis. (A) Experimental design. (B) Representative confocal images of neuronal cell bodies labeled with Dex (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 20 μm. (C, D) Number of Dex and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (C) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 50.83, p < 0.0001. (D) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 27.15, p < 0.0001. (E) Experimental design. (F) Representative confocal images of neuronal cell bodies labeled with Tf (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 20 μm. (G, H) Number of Tf and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (G) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 49.51, p < 0.0001. (H) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 29.31, p < 0.0001. For C, D and G, H: data are shown as box and whisker plots with each dot representing one individual neuron; results from Dunn's multiple comparisons test are displayed on the figure; data from 3 independent cultures.

Article Snippet: After blocking with 3% BSA, primary antibodies against GFP (1:150) (Invitrogen, Cat. No. A11122, RRID:AB_221569), Syn1 (1:500) (Synaptic Systems, Cat. No. 106011, RRID:AB_10805139), Syp1 (1:500) (Synaptic Systems, Cat. No. 101006, RRID: AB_2622239), and/or MAP2 (1:1000) (Synaptic Systems, Cat. No. 188004, RRID:AB_2138181) were diluted in blocking buffer and incubated overnight at 4°C in a humid chamber.

Techniques: Labeling, Negative Control, Whisker Assay

Journal: Journal of Neurochemistry

Article Title: Synaptobrevin‐2 Containing Extracellular Vesicles Are Rapidly Incorporated Into Mammalian Neurons via a Dynamin‐Dependent Pathway

doi: 10.1111/jnc.70551

Figure Lengend Snippet: Syb2‐containing neuronal EV uptake in the axonal compartment is Dynamin‐dependent and occurs independently of macropinocytosis. (A) Experimental design. (B) Representative confocal images of synapses labeled with Dex (yellow), immunofluorescently labeled with anti‐Syp1 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 5 μm. (C, D) Number of Dex and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (C) Kruskal‐Wallis non‐parametric test, Kruskal–Wallis statistic = 48.26, p < 0.0001. (D) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 46.09, p < 0.0001. (E) Experimental design. (F) Representative confocal images of neuronal cell bodies labeled with Tf (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 5 μm. (G, H) Number of Tf and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (G) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 35.22, p < 0.0001. (D) Kruskal‐Wallis non‐parametric test, Kruskal–Wallis statistic = 30.51, p < 0.0001. For C, D and G, H: data are shown as box and whisker plots and each dot is the average of all presynaptic boutons quantified in one image; results from Dunn's multiple comparisons test are displayed on the figure; data from 3 independent cultures.

Article Snippet: After blocking with 3% BSA, primary antibodies against GFP (1:150) (Invitrogen, Cat. No. A11122, RRID:AB_221569), Syn1 (1:500) (Synaptic Systems, Cat. No. 106011, RRID:AB_10805139), Syp1 (1:500) (Synaptic Systems, Cat. No. 101006, RRID: AB_2622239), and/or MAP2 (1:1000) (Synaptic Systems, Cat. No. 188004, RRID:AB_2138181) were diluted in blocking buffer and incubated overnight at 4°C in a humid chamber.

Techniques: Labeling, Negative Control, Whisker Assay

Journal: Cell reports

Article Title: Pleiotrophin regulates presynaptic assembly and function through heparan sulfate-dependent binding to neurexin1

doi: 10.1016/j.celrep.2026.117827

Figure Lengend Snippet:

Article Snippet: Chicken anti-MAP2 , Synaptic Systems , Cat# 188006; RRID:AB_2619881.

Techniques: Virus, Recombinant, Magnetic Beads, Mass Spectrometry, shRNA, Sequencing, Software

Journal: Cell Reports

Article Title: Striatal neuron dysfunction in C9ORF72-FTD/ALS is driven by AIS and potassium channel dysregulation

doi: 10.1016/j.celrep.2026.117672

Figure Lengend Snippet: Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

Article Snippet: MAP2 anti-Rabbit (1:1000) , Synaptic Systems , Cat# 188 003; RRID AB_2281442.

Techniques: Staining, Marker